Journal of General Virology (1995) 76, 1497-1501

F.J. Legorburu, D.J. Robinson, L. Torrance and G.H. Duncan (1995)
Antigenic analysis of nematode-transmissible and non-transmissible isolates of tobacco rattle tobravirus using monoclonal antibodies
Journal of General Virology 76 (6), 1497-1501
Abstract: Murine monoclonal antibodies (MAbs) were produced against a nematode non-transmissible isolate of tobacco rattle tobravirus (TRV-PLB). Two of the MAbs (SCR 80 and 81) did not react with the serologically closely related isolate TRV-PPK20 (both isolates belong to the PRN serotype), which is readily transmissible by nematodes. When further isolates of the PRN serotype were tested, all the non-transmissible ones reacted with these two MAbs, but so also did some of those that are readily transmissible. SCR 80 and 81 were able to trap TRV-PLB particles onto electron microscope grids and reacted with metatopes located near the C terminus of the particle protein. The epitope recognized by SCR 81 was discontinuous. The MAbs that reacted with both TRV-PLB and TRV-PPK20 recognized either continuous cryptotopes (SCR 78) or discontinuous neotopes (SCR 79 and 82).
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Lesley Torrance

Research topic(s) for pests/diseases/weeds:
molecular biology - genes
transmission/dispersal of plant diseases

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Tobacco rattle virus