Journal of General Virology (1993) 74, 2661-2668

M. Kool, J.T. Voeten, R.W. Goldbach, J. Tramper and J.M. Vlak (1993)
Identification of seven putative origins of Autographa californica multiple nucleocapsid nuclear polyhedrosis virus DNA replication
Journal of General Virology 74 (12), 2661-2668
Abstract: Seven putative origins of DNA replication (oris) were identified and located on the genome of Autographa californica multiple nucleocapsid nuclear polyhedrosis virus (AcMNPV), when an improved infection- dependent replication assay was used. A threefold higher yield of amplified plasmid was achieved when an m.o.i. of 1 was used (instead of 25), and another twofold increase was obtained when the interval between transfection and infection was extended from 5 to 24 h. Six of the putative oris were located in hr regions with homologous sequences. This suggests that all hrs in AcMNPV are bifunctional, i.e. have both ori and enhancer activity for transcription. In addition to the six hrs, the HindIII-K fragment of AcMNPV was also identified to carry a putative ori, although this fragment does not contain an hr region. However, the individual role of these seven oris during viral DNA replication, and whether they are all active simultaneously in vivo, is still unclear. The replication of an ori-containing plasmid starts at the same time (6 h post-infection) and proceeds at the same rate as viral DNA replication. A circular topology of ori-containing plasmids was a prerequisite for replication. Linear DNA, with an ori, did not replicate. Therefore, we suggest a theta structure or a rolling-circle as a model for baculovirus DNA replication.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Just M. Vlak

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
Alphabaculovirus aucalifonicae (entomopathogen)