Journal of General Virology (1989) 70, 1017-1024

Allan M. Crawford (1989)
Engineering of an Oryctes baculovirus recombinant: Insertion of the polyhedrin gene from the Autographa californica nuclear polyhedrosis virus
Journal of General Virology 70 (4), 1017-1024
Abstract: A cotransfection method has been developed for the generation of recombinant Oryctes baculoviruses. The permissive coleopteran cell-line DSIR-HA-1179 was transfected with a mixture of Oryctes baculovirus DNA (strain PV505) and a transfer vector. The transfer vector, a pUC8-based plasmid, contained the polyhedrin gene from the Autographa californica nuclear polyhedrosis virus, flanked by Oryctes baculovirus DNA from the HindIII fragment N. Oryctes baculovirus does not form plaques with DSIR-HA-1179 cells. Therefore an endpoint dilution method was used to screen for, recover and purify recombinants. There was no phenotypic character that could be used for detecting recombinants, so a dot blot assay was used to screen infected cultures for the presence of recombinants. A recombinant generated by this method contained the entire polyhedrin gene inserted at 98.03 map units from the designated start of the Oryctes baculovirus physical map. No evidence of transcription or translation of the polyhedrin gene was obtained.
(The abstract has been supplied by the author or ISPI and is excluded from the Creative Commons licence.)
(original language: English)
Link to article at publishers website
Database assignments for author(s): Allan M. Crawford

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
Alphanudivirus oryrhinocerotis (entomopathogen)