Journal of General Virology (1985) 66, 529-539

Allan M. Crawford and Cathy Sheehan (1985)
Replication of Oryctes baculovirus in cell culture: Viral morphogenesis, infectivity and protein synthesis
Journal of General Virology 66 (3), 529-539
Abstract: The sequence of events in the replication of Oryctes baculovirus in DSIR-HA-1179 cells began with the uptake of virus particles by pinocytosis at the plasma membrane. Seven h after infection, enveloped virus particles were observed inside a cleared area in the nucleus. Virus was released from cells by budding through the plasma membrane where a second unit membrane was acquired. The infectivity of virus particles with a single envelope purified from within cells was not significantly different from that of virus which had acquired the second envelope by budding. Twenty-seven structural virus proteins were identified by gradient polyacrylamide gel electrophoresis. The synthesis of eight of the major structural proteins was detected by pulse labelling infected cells with l-[³⁵S]methionine. No late protein synthesis, as observed in subgroup A baculoviruses, was detected in this subgroup C baculovirus.
(The abstract has been supplied by the author or ISPI and is excluded from the Creative Commons licence.)
(original language: English)
Link to article at publishers website
Database assignments for author(s): Allan M. Crawford

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
rearing/culturing/mass production
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
Alphanudivirus oryrhinocerotis (entomopathogen)