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Journal of General Plant Pathology (2013) 79, 243-248

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Hirotaka Yamaguchi, Jun Ohnishi, Koji Miyatake, Tsukasa Nunome, Akio Ohyama, Satomi Negoro and Hiroyuki Fukuoka (2013)
A simple, efficient agroinoculation soaking procedure for Tomato yellow leaf curl virus
Journal of General Plant Pathology 79 (4), 243-248
Abstract: Tomato yellow leaf curl virus (TYLCV, genus Begomovirus, family Geminiviridae), poses a serious threat to tomato crops in tropical and subtropical regions. We developed a simple agroinoculation method with an infectious clone of TYLCV. Dipping of excised sections of susceptible tomato shoots in an Agrobacterium suspension successfully introduced the replicating virus with high efficiency. An additional vacuum treatment for 5 min ensured uniform infection without escapes, allowing evaluation of differences in TYLCV resistance among tomato cultivars. The method can be used in laboratory experiments for virological studies and in breeding programs for resistant cultivars.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Jun Ohnishi

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution
resistance/tolerance/defence of host


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Tomato yellow leaf curl virus Tomato (Solanum lycopersicum)