Journal of General Plant Pathology (2011) 77, 304-306

Masamichi Isogai, Tatuto Nakamura, Koji Ishii, Manabu Watanabe, Noriko Yamagishi and Nobuyuki Yoshikawa (2011)
Histochemical detection of Blueberry latent virus in highbush blueberry plant
Journal of General Plant Pathology 77 (5), 304-306
Abstract: Blueberry latent virus (BBLV) was detected in 27 of 95 asymptomatic highbush blueberry trees in a blueberry field in Japan. In situ hybridization showed that the viral RNAs were detected in the palisade mesophyll, spongy mesophyll and vascular bundle of leaves. In western blot analysis, an antibody to the viral protein encoded by open reading frame 1 (ORF1) reacted with a 48-kDa protein specific for blueberry trees in which BBLV was detected. Immunogold electron microscopy revealed that amorphous bodies in the cytoplasm of blueberry cells were labeled with antibodies to the ORF1 protein.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Masamichi Isogai, Nobuyuki Yoshikawa

Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Amalgavirus vaccinii	Blueberry/cranberry (Vaccinium)	Japan