Journal of General Plant Pathology (2004) 70, 215-217

Yukiko Maeda, Akinori Kiba, Kouhei Ohnishi and Yasufumi Hikichi (2004)
New method to detect oxolinic acid-resistant Burkholderia glumae infesting rice seeds using a mismatch amplification mutation assay polymerase chain reaction
Journal of General Plant Pathology 70 (4), 215-217
Abstract: Bacterial seedling rot and grain rot of rice caused by Burkholderia glumae are seed-borne diseases, traditionally controlled with oxolinic acid (OA). Ser83Arg and Ser83Ile substitutions in GyrA protein are commonly responsible for moderate and high resistance to OA, respectively, in field isolates of B. glumae. To detect OA-resistant B. glumae infesting rice seeds, a mismatch amplification mutation assay polymerase chain reaction protocol was developed using DNA from bacteria incubated in modified S-PG medium and primers based on the amino acid substitutions at position 83 in GyrA.
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Link to article at publishers website

Research topic(s) for pests/diseases/weeds:
pesticide resistance of pest

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Burkholderia glumae	Rice (Oryza)