Journal of Economic Entomology (2022) 115, 2075-2082

Despoina Ev Kapantaidaki, Afroditi Krokida, Vasiliki Evangelou, Panagiotis Milonas and Dimitrios P. Papachristos (2022)
A molecular diagnostic assay for the discrimination of aphid species (Hemiptera: Aphididae) infesting citrus
Journal of Economic Entomology 115 (6), 2075-2082
Abstract: Aphid species (Hemiptera: Aphididae) are among the most serious pests for citrus cultivation throughout the world causing substantial crop damages. Accurate identification of aphids to the species level can be difficult, though being crucial for their effective management. In this study, a molecular diagnostic assay for distinguishing eleven aphid species was developed. A fragment of the mitochondrial Cytochrome Oxidase I (mtCOI) gene was used and a Polymerase Chain Reaction–Restriction Fragment Length Polymorphism (PCR–FLP) analysis with five restriction enzymes, based on DNA sequence polymorphisms, was applied to differentiate the eleven aphid species. This molecular technique allows aphid species at any life stage to be discriminated accurately and simply and can be a useful tool for monitoring the populations of economically important aphid species.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Panagiotis G. Milonas, Dimitrios P. Papachristos

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Aphis citricidus	Citrus (genus)