Journal of Economic Entomology (2016) 109, 815-820

Ryan W. Geisert, Mark R. Ellersieck and Bruce E. Hibbard (2016)
Tolerance of eCry3.1Ab in reciprocal cross offspring of eCry3.1Ab-selected and control colonies of Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae)
Journal of Economic Entomology 109 (2), 815-820
Abstract: Two new insect colonies were created by separating virgin western corn rootworm, Diabrotica virgifera virgifera LeConte, males and females from both a selected laboratory colony that was being reared on eCry3.1Ab-expressing corn (Zea mays L.) and a control colony reared on its near-isoline corn. Females from the selected colony were paired with males of the control colony and vice versa to create both a selected female by control male colony (Sel-female) and control female by selected male colony (Con-female). Both colonies along with their parental colonies (eCry3.1Ab-selected and control) were evaluated on eCry3.1Ab-expressing corn and its near-isoline in seedling assays. Larvae from each colony were also used in diet toxicity experiments in order to determine the LC₅₀ and EC₅₀ values for the eCry3.1Ab toxin for each. Statistical analysis of seedling assay experiments did not indicate any significant colony × corn interaction but did show a significant main effect of corn type for both larval recovery and larval head capsule widths. Results from the diet toxicity assays showed the control colony to have a significantly lower LC₅₀ value than the selected and cross colonies and a significantly lower EC₅₀ than the selected and Con-female colonies. Calculations of dominance values (h) of eCry3.1Ab resistance traits from seedling assays indicated that the two reciprocal cross colonies have a dominance value (h) of ~1, suggesting dominance of the eCry3.1Ab resistance trait.
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Link to article at publishers website
Database assignments for author(s): Bruce E. Hibbard

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
resistance/tolerance/defence of host

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Diabrotica virgifera
Bacillus thuringiensis Cry3A-toxin (entomopathogen)	Diabrotica virgifera