Journal of Applied Microbiology (2013) 114, 219-228

D. Mejri, E. Gamalero and T. Souissi (2013)
Formulation development of the deleterious rhizobacterium Pseudomonas trivialis X33d for biocontrol of brome (Bromus diandrus) in durum wheat
Journal of Applied Microbiology 114 (1), 219-228
Abstract:
Aim
To develop an appropriate formulation of the deleterious rhizobacterium Pseudomonas trivialis X33d and to evaluate its effectiveness to reduce brome growth.
Methods and Results
Two formulations of Ps. trivialis X33d, a semolina-kaolin granular formulation (Pesta) and talc-kaolin powder, were prepared and their effectiveness in reducing brome growth was evaluated. Both brome suppression and cell viability of X33d were higher in Pesta granular formulation than in talc-kaolin powder one. The impact of storage temperature and the addition of adjuvants (sucrose and oil) to the granular formulation of X33d were assessed in order to improve the shelf life of the formulation. The longest viability was found in formulated product supplemented with adjuvants and stored at 4°C. The effect of Pesta granules supplemented with adjuvants and stored for 6 months at 4°C on brome and wheat growth under controlled and greenhouse conditions was evaluated. The X33d formulation in Pesta increased the growth of wheat and reduced brome growth.
Conclusion
Our results indicate that Ps. trivialis X33d formulated in Pesta has potential as a bioherbicide to control brome.
Significance and Impact of the Study
Because of the impracticality of applying bacterial cell suspension on a large scale, the use of Pesta granules of X33d against brome could help in achieving a sustainable agriculture application of a bioherbicide.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Thouraya Souissi

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
formulation/storage of bioagents
evaluation - screening - selection

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Bromus diandrus (weed)	Wheat (Triticum)	Tunisia
Pseudomonas trivialis (weed pathogen)	Bromus diandrus (weed)	Wheat (Triticum)	Tunisia