Journal of Applied Microbiology (2003) 95, 301-309

M. Abadias, N. Teixidó, J. Usall and I. Viñas (2003)
Optimization of growth conditions of the postharvest biocontrol agent Candida sake CPA-1 in a lab-scale fermenter
Journal of Applied Microbiology 95 (2), 301-309
Abstract: Aim:To maximize the growth (expressed as number of viable cells per millilitre) of the postharvest biocontrol agent Candida sake CPA-1 at laboratory scale conditions.
Methods and Results:Growth conditons (aeration, agitation speed and inoculum size) were studied in batch conditions in a 5 l fermenter using molasses and urea as growth medium. Consumption of sugars and urea were analysed. Fed-batch studies were also carried out. Glucose and fructose were consumed during the exponential growth phase and were depleted after 18 h of growth. On the contrary, C. sake cells assimilated sucrose during the stationary phase. There was not growth improvement when fed-batch technology was used. Addition of an extra amount of glucose or molasses after 18 h of growth did not contribute to increase final population.
Conclusions:Maximum growth (about 8 x 10⁸ CFU ml^-1) was obtained at batch fermentation after 30 h growth at 400 rev min^-1, 150 l h^-1 of air and initial concentration of 10⁶ CFU ml^-1.
Significance and Impact of the Study:The results obtained in this study are an approach for further upscaling of C. sake production.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Maribel Abadias, Josep Usall, Neus Teixidó

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
rearing/culturing/mass production

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
Candida sake (antagonist)