Journal of Applied Entomology (2015) 139, 638-645

S. Shin, S. Jung, K. Heller, F. Menzel, T.K. Hong, J.S. Shin, S.H. Lee, H. Lee and S. Lee (2015)
DNA barcoding of Bradysia (Diptera: Sciaridae) for detection of the immature stages on agricultural crops
Journal of Applied Entomology 139 (8), 638-645
Abstract: We investigated the usefulness of mitochondrial cytochrome c oxidase (COI) DNA barcoding of the genus Bradysia for the detection of immature stages and cryptic species complex. Although the larvae of some species in this genus are agricultural pests, immature stages are rarely identified due to the lack of key morphological characteristics. We constructed partial sequences of the COI gene for 25 species of Bradysia as a first step towards a DNA barcode. Using these data, Bradysia impatiens, B. procera and B. peraffinis were identified from larval specimens collected, respectively, from paprika, ginseng and oak sawdust beds used for cultivating shiitake. Our findings reveal a complex of three species within the B. tilicola group. These species were all identified as important pest B. ocellaris based on the morphology of male genital structures; however, the interspecific genetic divergence of the COI region was significantly greater (16.1–19.4%) than the intraspecific variation in each species. Therefore, B. ocellaris may consist of at least three species. The results demonstrate that COI DNA barcodes are useful for Bradysia species identification.
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Link to article at publishers website
Database assignments for author(s): Seung Hwan Lee

Research topic(s) for pests/diseases/weeds:
identification/taxonomy

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Bradysia ocellaris
	Bradysia impatiens