Journal of Applied Entomology (2012) 136, 530-538

Y. Gómez-Bonilla, M. López-Ferber, P. Caballero, X. Léry and D. Muñoz (2012)
Costa Rican soils contain highly insecticidal granulovirus strains against Phthorimaea operculella and Tecia solanivora
Journal of Applied Entomology 136 (7), 530-538
Abstract: The aim of this work was to isolate and characterize novel Phthorimaea operculella granulovirus (PhopGV) strains from Costa Rican soils. Three novel strains, named PhopGV-CR3, PhopGV-CR4 and PhopGV-CR5, were isolated from three locations in Costa Rica, Alvarado, Zarcero and Abangares, respectively, by means of soaking potato tubers with diluted soil samples. An additional strain, PhopGV-CR2, was identified from diseased larvae from a Tecia solanivora laboratory culture. Restriction fragment length polymorphisms obtained for each isolate with six restriction endonucleases (RENs) allowed their identification as four distinct PhopGV strains. Both REN and Polymerase chain reaction analyses indicated the existence of an array of genotypic variants present in all isolates. Bioassays in P. operculella and T. solanivora showed that PhopGV-CR3 was well adapted to the two coexisting hosts with high levels of pathogenicity against both pest species. The mean lethal dose values of this strain were 2.8 OBs/mm² for P. operculella and less than 0.5 OBs/mm² for T. solanivora. We conclude that PhopGV-CR3 shows great promise for soil application against these pests in Costa Rican potato crops.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Delia Muñoz, Primitivo Caballero

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
surveys/distribution/isolation

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Phthorimaea operculella	Potato (Solanum tuberosum)	Costa Rica
	Tecia solanivora	Potato (Solanum tuberosum)	Costa Rica
Betabaculovirus phoperculellae (entomopathogen)	Phthorimaea operculella		Costa Rica
Betabaculovirus phoperculellae (entomopathogen)	Tecia solanivora		Costa Rica