Journal of Applied Entomology (2003) 127, 258-264

R. Baumann, R. Schubert, W. Heitland, M.-A. Auger-Rozenberg, P. Faivre-Rimpant and G. Müller-Starck (2003)
Genetic diversity within and among populations of Diprion pini (Hym., Diprionidae) determined by random amplified polymorphic DNA-polymerase chain reaction of haploid males
Journal of Applied Entomology 127 (5), 258-264
Abstract: Based on haploid males, random amplified polymorphic DNA (RAPD) markers were used to study genetic variation within and among four French populations and one Finnish outgroup population of the common pine sawfly, Diprion pini (L.), representing a severe European forest pest associated with mass outbreaks. Taking into account that all multilocus haplotypes were detected, a total of 140 individuals were completely discriminated by means of 17 polymorphic markers (present or absent), which were amplified from nine selected random-decamer primers. All populations shared the same genetic types, but pronounced population-specific frequency distributions were found, indicating that on average 84% of the present genetic variation exists within populations and the remaining smaller part counts for interpopulational variation. The haplotype differentiation registered was able to distinguish between the class of lowland populations and the class of highland populations. Reproductive isolation and therefore limited altitudinal gene flow, indicated in case of a French highland population by an enlarged number of fixed markers, and hitchhiking effects with respect to selective processes at certain loci following local adaptation and speciation are discussed to explain the population structures found. A possible selection is indicated by five markers, showing significantly different frequency distributions between the class of highland populations and the class of lowland populations.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Werner Heitland

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Diprion pini