Japanese Journal of Applied Entomology and Zoology (2011) 55, 49-57

Shinji Isayama, Takeshi Suzuki, Madoka Nakai and Yasuhisa Kunimi (2011)
[Influences of tannic acid and polyphenols in the leaves of strawberry, Fragaria×ananassa, and Perilla, Perilla frutescens viridis on the insecticidal activity of Bacillus thuringiensis formulation against the common cutworm, Spodoptera litura (Lepidoptera: Noctuidae)]
Japanese Journal of Applied Entomology and Zoology 55 (2), 49-57
Abstract: It is known that insecticidal activity of Bacillus thuringiensis sp. aizawai formulation (BT) against common cutworm (Spodoptera litura) is reduced on the leaves of strawberry (Fragaria×ananassa) and perilla (Perilla frutescens viridis). Since some polyphenols in plants might be the cause of this phenomenon, we examined how various polyphenols influence BT activity. First, we evaluated the influence of tannic acid and garlic acid as representative polyphenols on the insecticidal activity of BT against the common cutworm. We also identified and quantified polyphenols contained in the leaves of strawberry and perilla and then evaluated their influence on the insecticidal activity of BT. The influences of tannic acid and garlic acid on insecticidal activity of BT were evaluated by oral administration to 3rd instar larvae of the common cutworm. Tannic acid showed dose-dependent inhibition of insecticidal activity, whereas garlic acid had no apparent influence on insecticidal activity. Ellagic acid, catechin, quercetin, p-coumaric acid and ferulic acid were identified as polyphenols contained in the leaves of strawberry. Rosmarinic acid, apigenin, luteorin, eugenol, p-coumaric acid and ferulic acid were identified in perilla. We then evaluated the influence of these polyphenols found in strawberry and perilla on the activity of BT using the same oral administration protocol. Catechin, quercetin, luteorin, apigenin, rosmarinic acid and p-coumaric acid suppressed the insecticidal activity of BT dose-dependently, whereas ellagic acid, eugenol and ferulic acid had no apparent influence. In particular, the influence of rosmarinic acid was highest among the polyphenols evaluated, followed by catechin. These results suggested that catechin in strawberry and rosmarinic acid in perilla were mainly responsible for inhibitory effects on the insecticidal activity of BT.
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(original language: Japanese)
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Database assignments for author(s): Madoka Nakai, Yasuhisa Kunimi

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
environment/habitat manipulation

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Spodoptera litura	Strawberry (Fragaria)
	Spodoptera litura	Perilla (Perilla frutescens)
Bacillus thuringiensis var. aizawai (entomopathogen)	Spodoptera litura	Strawberry (Fragaria)
Bacillus thuringiensis var. aizawai (entomopathogen)	Spodoptera litura	Perilla (Perilla frutescens)