International Journal of Systematic and Evolutionary Microbiology (2015) 65, 2761-2765

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Daniela Flôres, Ana Paula de Oliveira Amaral Mello, Thays Benites Camargo Pereira, Jorge Alberto Marques Rezende and Ivan Paulo Bedendo (2015)
A novel subgroup 16SrVII-D phytoplasma identified in association with erigeron witches' broom
International Journal of Systematic and Evolutionary Microbiology 65 (8), 2761-2765
Abstract: Erigeron sp. plants showing symptoms of witches' broom and stunting were found near orchards of passion fruit in São Paulo state, Brazil. These symptoms were indicative of infection by phytoplasmas. Thus, the aim of this study was to detect and identify possible phytoplasmas associated with diseased plants. Total DNA was extracted from symptomatic and asymptomatic plants and used in nested PCR conducted with the primer pairs P1/Tint and R16F2n/16R2. Amplification of genomic fragments of 1.2kb from the 16S rRNA gene confirmed the presence of phytoplasma in all symptomatic samples. The sequence identity scores between the 16S rRNA gene of the phytoplasma strain identified in the current study and those of previously reported ' Candidatus Phytoplasma fraxini'-related strains ranged from 98 % to 99 % indicating the phytoplasma to be a strain affiliated with ' Candidatus Phytoplasma fraxini'. The results from a phylogenetic analysis and virtual RFLP analysis of the 16S rRNA gene sequence with 17 restriction enzymes revealed that the phytoplasma strain belongs to the ash yellows phytoplasma group (16SrVII); the similarity coefficient of RFLP patterns further suggested that the phytoplasma represents a novel subgroup, designated 16SrVII-D. The representative of this new subgroup was named EboWB phytoplasma (Erigeron bonariensis Witches' Broom).
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Ivan Paulo Bedendo

Research topic(s) for pests/diseases/weeds:
identification/taxonomy

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


16SrVII phytoplasma group Erigeron (crop) Brazil (south)
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