Insect Science (2005) 12, 331-334

Ya-Jie Ji, Yu-Chun Wu and De-Xing Zhang (2005)
Novel polymorphic microsatellite markers developed in the cotton bollworm Helicoverpa armigera (Lepidoptera: Noctuidae)
Insect Science 12 (5), 331-334
Abstract: A novel set of five polymorphic di- or trinucleotide microsatellite loci suitable for population genetic study were developed from an enriched genomic library for the pest insect cotton bollworm, Helicoverpa armigera, and cross-amplifiability of these and other published loci was tested in a closely related species, the tobacco budworm, H. assulta. The expected heterozygosity at these loci ranges from 0.62 to 0.91 in the cotton bollworm. The observed allele numbers varies from 4 to 12 in the limited number of individuals tested. Although a large proportion of cloned microsatellite sequences are present in multi-copy in the cotton bollworm genome, the overwhelming majority of the finalized polymorphic diallelic loci are tri-nucleotide microsatellites - an unexpected outcome, which should facilitate subsequent genotyping analysis.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Helicoverpa armigera
	Helicoverpa assulta