Gesunde Pflanzen (2007) 59, 101-106

Luitgardis Seigner, Dorothee Kaemmerer and Margaretha Kappen (2007)
Realtime-PCR zum Nachweis von Clavibacter michiganensis subsp. sepedonicus und Ralstonia solanacearum - Grundlagen, Methode und praktische Hinweise
[Realtime-PCR for detection of Clavibacter michiganensis subsp. sepedonicus und Ralstonia solanacearum - Basic information, method and practical advice]
Gesunde Pflanzen 59 (3), 101-106
Abstract: Realtime-PCR as a quantitative nucleic acid amplification method is an improvement of conventional PCR, which only provides qualitative results. In the meantime Realtime-PCR, which is fast, sensitive and specific as well, is increasingly used for detection of plant pathogens. In this report basic knowledge about Realtime-PCR is presented. Furthermore the method established at the Bavarian State Research Center for Agriculture (LfL) for detection of Bacterial Ring Rot and Brown Rot Pathogens of potato is described to assist diagnostic laboratories which want to apply this technique.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: German)
Link to article at publishers website
Database assignments for author(s): Luitgardis Seigner

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Clavibacter sepedonicus	Potato (Solanum tuberosum)
	Ralstonia solanacearum	Potato (Solanum tuberosum)