Forest Pathology (2007) 37, 403-408

L. Belbahri, G. Calmin, S. Wagner, E. Moralejo, S. Woodward and F. Lefort (2007)
Specific hybridization real-time PCR probes for Phytophthora ramorum detection and diagnosis
Forest Pathology 37 (6), 403-408
Abstract: Sudden Oak Death, caused by Phytophthora ramorum, poses a serious threat to native American oaks, and is also present in Europe where it has been isolated from numerous European ornamental plant nurseries. Its proven aggressiveness against plants in the Fagaceae and Ericaceae and the damage it has caused in North America have lead to it being assigned quarantine status. The timely and accurate detection of P. ramorum is a critical aid in the study of the epidemiology and biology of this pathogen. As a regulated organism, the availability of a sensitive and reliable assay is essential when attempting to achieve early detection of the pathogen. In this work, new specific hybridization probes for a real-time PCR amplification method were found to be rapid, robust and labour-saving, and proved suitable for routine use in a molecular diagnostic laboratory.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Eduardo Moralejo, Stephen Woodward

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Phytophthora ramorum