Florida Entomologist (2018) 101, 505-507

Marie-Claude Bon, Fatiha Guermache, Daniele de Simone, Massimo Cristofaro, Ann Vacek and John Goolsby (2018)
Insights into the microbes and nematodes hosted by pupae of the arundo leaf miner, Lasioptera donacis (Diptera: Cecidomyiidae)
Florida Entomologist 101 (3), 505-507
Abstract: The leaf miner Lasioptera donacis Coutin (Diptera: Cecidomyiidae) is a biological control agent of the invasive weed, Arundo donax L. (Poaceae), that was approved for release in the U.S. and Mexico. Pupae are preferred for shipment of living material to quarantine facilities. There is a question of whether emerged females would carry conidia of a potential mutualist fungus, and in particular the saprophyte Arthrinium arundinis, from the pupa or if they would have to acquire the conidia exclusively from the plant to start the oviposition process. We artificially smeared leaf-sheaths of growing plants with A. arundinis before being exposed to female midges, and maintained these host plants until the pupal stage of the midge developed. Polymerase chain reaction methods were applied to detect A. arundinis and any other potential fungi in these pupae. Only 9% of the pupae were infested by fungi or yeast, predominantly belonging to the genus Sarocladium, but not A. arundinis, confirming that the newly emerged females are free of this fungus and will have to acquire conidia present on the leaf-sheath for successful oviposition. We also tentatively tested by PCR for the presence of parasitic nematodes in these pupae. More than 42% of the pupae were shown to be infested specifically by T. gyraloura. Such high infection rate calls for developing methods to eliminate this parasite or to find a parasitefree native population prior to release of L. donacis adults in North America for biological control of A. donax.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Full text of article
Database assignments for author(s): Marie-Claude Bon, John A. Goolsby

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Arundo donax (weed)		France
Lasioptera donacis (weed bioagent)	Arundo donax (weed)		France