Fitopatologia Brasileira (2007) 32, 253-256

Ely O. Garcia, Marcos V. Casagrande, Alejandro M. Rago and Nelson S. Massola Jr. (2007)
Método para inoculação de ferrugem da cana-de-açúcar em segmentos de folhas
[An inoculation method for sugarcane rust in leave segments]
Fitopatologia Brasileira 32 (3), 253-256
Abstract: The reaction of sugarcane to Puccinia melanocephala Syd. and P. Syd. populations is intensely influenced by environmental conditions, make it difficult to conduct field evaluations of genotypes. This work aimed to develop a method for rust inoculation in leaf segments. The segments were obtained from 30-day-old plants of the susceptible variety SP70-1143. These were inoculated (2 x 10⁴ viable spores/mL) and kept in test tubes. The treatments consisted of different incubation conditions, considering the variables (i) use or not of wet chamber after the inoculation; (ii) immersion of ¼ or ¾ of the section in the liquid contained in the test tube and; (iii) addition or not of benzimidazole in the water of the tube. The best treatment consisted of immersion of ¾ of the section in test tubes containing water, with no moist chamber after the inoculation. After incubation (21 °C ± 1ºC, 12 h of photoperiod) the sections remained viable for 20 days and showed good rust development. The method showed similar results when compared to the inoculation of whole plants of 3 varieties, making it possible to discriminate efficiently their reactions to rust.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Portuguese)
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Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Puccinia melanocephala	Sugarcane (Saccharum officinarum)