Fitopatologia Brasileira (2005) 30, 650-654

Ana R.P. Nascimento, Rosa L.R. Mariano and Marco A.S. Gama (2005)
Métodos de preservação e crescimento de Xanthomonas campestris pv. viticola em meio de cultura variando temperatura, pH e concentração de NaCl
[Preservation methods and growth of Xanthomonas campestris pv. viticola in culture medium varying temperature, pH and NaCl concentration]
Fitopatologia Brasileira 30 (6), 650-654
Abstract: The phytopathogenic bacterium Xanthomonas campestris pv. viticola (Xcv) induces grapevine (Vitis vinifera) bacterial canker, causing severe losses in Brazil. Four preservation methods [dried paper strips (DPS), periodic transfer (PT), sterile distiled water (SDW) and dried leaves (DL)] were compared for storing two Xcv strains over a 12-month periods. Viability and pathogenicity were evaluated every month and estimated by bacterial growth and area under the disease incidence curve (AUDIC). Both the DPS and SDW methods maintained 100% of cell viability to and showed higher AUDIC values for 11 months. The PT did not permit growth at 30 days while DL maintained cell viability for up to five months. The growth of two Xcv strains in liquid culture medium at varying temperatures (0, 5, 10, 15, 20, 25, 27, 28, 29, 30, 35, 40 and 45°C), pH (5.0; 5.,5; 6.0; 6.5; 7.0; 7.5; 8.0; 8.5 and 9.0) and NaCl concentration (1, 2, 3, 4, 5, 6 and 7%) was evaluated with spectrophotometer. The Xcv growth was observed from 5 to 35 °C, with optimum growth from 27 to 29 °C. The Xcv did not grow at zero and 40 °C. The optimum pH for Xcv growth was 7.5. The pathogen growth declined from 3.0% NaCl and was null at 6.0%.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Portuguese)
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Database assignments for author(s): Rosa de Lima R. Mariano

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution
rearing/culturing/mass production

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Xanthomonas citri pv. viticola	Grapevine (Vitis)	Brazil (NE)
	Xanthomonas campestris