Fitopatologia Brasileira (2004) 29, 670-675

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Marcelo Eiras, Alexandre L.R. Chaves, Silvia R. Moreira, Jansen de Araujo and Addolorata Colariccio (2004)
Caracterização de um isolado do Pepper mild mottle virus que não quebra a resistência do gene L3 em Capsicum sp.
[Characterization of a non-L3 gene-resistance breaking Pepper mild mottle virus isolate in Capsicum]
Fitopatologia Brasileira 29 (6), 670-675
Abstract: A strain of Pepper mild mottle virus (PMMoV) was identified by biological and serological analysis of pepper (Capsicum baccatum) 'Dedo de Moça' seeds from São Paulo, SP, Brazil. To confirm these results, RT-PCR was performed with specific primers flanking the coat protein (CP) ORF of tobamoviruses in subgroup 1. The DNA-amplified fragments, when sequenced and compared with other tobamovirus CP sequences stored in the GenBank, showed nucleotide similarity between 94 to 100% with other PMMoV CP sequences, and below 75% with the subgroup I tobamovirus species (Tobacco mosaic virus, Tomato mosaic virus and Odontoglossum ringspot virus) and 65% with species in the subgroups II and III. The Brazilian isolate showed 100% identity with a Japanese isolate, strongly suggesting that this pathogen could have been introduced from that country. This fact confirms the importance of the isolate's characterization, in order to establish adequate control measures to guarantee healthy seed interchange and to avoid the introduction and spread of tobamoviruses into new growing areas. The deduced CP aminoacids sequence showed that the PMMoV-BR was not capable to overcome L3 gene resistance. This fact was confirmed by the inoculation and reaction of differential Capsicum species. The Brazilian isolate was not able to infect C. chinense (L3) and C. chacoense (L4).
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Portuguese)
Full text of article
Database assignments for author(s): Addolorata Colariccio, Marcelo Eiras

Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Pepper mild mottle virus Green pepper/chilli (Capsicum) Brazil (south)
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