Fitopatologia Brasileira (2003) 28, 298-301

Adriane Wendland, Daniela Truffi, Rui P. Leite Júnior and Luis E.A. Camargo (2003)
Seqüenciamento e variabilidade do fragmento genômico de Xylella fastidiosa amplificado pelos iniciadores RST31/33
[Sequencing and variability of the Xylella fastidiosa - specific genomic fragment amplified by the primer pair RST 31/33]
Fitopatologia Brasileira 28 (3), 298-301
Abstract: Xylella fastidiosa causes several plant diseases of economic importance such as citrus (Citrus spp.) variegated chlorosis (CVC), Pierce's disease of grapevine (Vitis vinifera) and leaf scorch of plum (Prunus salicina) and coffee (Coffea arabica). The nucleotide sequence of the genomic fragment, specific to Xylella fastidiosa, amplified by the pair of primers RST 31/33, was determined for 38 isolates from citrus and for isolates from grapevine, coffee and plum in order to assess the level of sequence polymorphism of this fragment among isolates, as well as its genomic identity. Sequence polymorphism was not observed among isolates from citrus, but was detected among isolates from citrus and from grapevine, coffee and plum. The presence of a RsaI restriction site, which distinguishes isolates from citrus and grapevine from plum and other arboreal species, was identified in a North American isolate from plum but not in a Brazilian one.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Portuguese)
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Database assignments for author(s): Luis E.A. Camargo

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Xylella fastidiosa	Citrus (genus)	Brazil (south)
	Xylella fastidiosa	Coffee (Coffea)	Brazil (south)
	Xylella fastidiosa	Prunus (other fruit tree species)	Brazil (south)