FEMS Microbiology Letters (2014) 357, 77-84

Zihao Chen, Tao Gao, Shuping Liang, Kexue Liu, Mingguo Zhou and Changjun Chen (2014)
Molecular mechanism of resistance of Fusarium fujikuroi to benzimidazole fungicides
FEMS Microbiology Letters 357 (1), 77-84
Abstract: Although carbendazim (MBC) and other benzimidazole fungicides have effectively controlled bakanae disease of rice (which is caused by Fusarium fujikuroi, F. proliferatum, and F. verticillioides) in the past, MBC resistance has become common. Previous research has shown that MBC resistance results from a mutation in the ß1-tubulin (ß1tub) gene in F. verticillioides. However, MBC resistance in F. fujikuroi, a predominant species in China, does not result from a mutation in the ß1tub. The molecular mechanism of F. fujikuroi resistance against benzimidazole fungicides is poorly understood. In this study, we determined that although ß1tub and ß2-tubulin (ß2tub) in F. fujikuroi have high homology with ß1tub and ß2tub in F. verticillioides, MBC resistance in F. fujikuroi results from mutations in ß2tub [GAG(Glu) -> GTG(Val) at codon 198, TTC(Phe) -> TAC(Tyr) at codon 200, and GGC(Gly) -> GGT(Gly) at codon 235] but not in ß1tub. Delta-ß2tub (ß2tub deletion) mutants were highly sensitive to MBC, produced fewer conidia and were less virulent than parental strains. Complementation of the Deltaß2tub mutants with a copy of the whole ß2tub locus from their parental strains restored the level of MBC resistance (or sensitivity) to that of the parental strain.
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Link to article at publishers website
Database assignments for author(s): Ming-Guo Zhou, Chang-Jun Chen

Research topic(s) for pests/diseases/weeds:
pesticide resistance of pest

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Fusarium fujikuroi	Rice (Oryza)