FEMS Microbiology Letters (2007) 271, 27-32

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Mesfin Bogale, Brenda D. Wingfield, Michael J. Wingfield and Emma T. Steenkamp (2007)
Species-specific primers for Fusarium redolens and a PCR-RFLP technique to distinguish among three clades of Fusarium oxysporum
FEMS Microbiology Letters 271 (1), 27-32
Abstract: The currently available morphological and molecular diagnostic techniques for Fusarium redolens and the three phylogenetic clades of Fusarium oxysporum are problematic. Aligned translation elongation factor 1-alpha (TEF-1-alpha) gene sequences from these species and their close relatives were used to design F. redolens-specific primers, and to identify restriction sites that discriminate among the three clades of F. oxysporum. The F. redolens-specific primers distinguished this species from all others included in the study. There were three TEF-1-alpha-RFLP patterns among formae speciales of F. oxysporum. These PCR-RFLP patterns corresponded with the three clades. These techniques provide simple and inexpensive diagnostic methods for the identification of F. redolens and members of the three clades of F. oxysporum.
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Database assignments for author(s): Mesfin Bogale, Brenda D. Wingfield, Michael J. Wingfield

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Fusarium oxysporum
Fusarium redolens