FEMS Microbiology Letters (2004) 231, 45-52

Xiaomin Hu, Bjarne Munk Hansen, Jørgen Eilenberg, Niels Bohse Hendriksen, Lasse Smidt, Zhiming Yuan and Gert Bolander Jensen (2004)
Conjugative transfer, stability and expression of a plasmid encoding a cry1Ac gene in Bacillus cereus group strains
FEMS Microbiology Letters 231 (1), 45-52
Abstract: The plasmid pHT73 containing cry1Ac and tagged with an erythromycin resistance gene was transferred from Bacillus thuringiensis subspecies kurstaki KT0 to several Bacillus cereus group strains by conjugation. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and phase contrast microscopy showed that the transconjugants containing plasmid pHT73 could express Cry1Ac toxin and produce bipyramidal crystalline inclusion bodies during sporulation. The study demonstrated that pHT73 could be transferred to B. thuringiensis subsp. kurstaki, several B. cereus strains and Bacillus mycoides. Under non-selective conditions, the stability of the pHT73 plasmid in the transconjugants was found to be 58.2-100% after 100 generations and 4-96% after 200 generations. The variations are mainly caused by the choice of receptor strain.
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Link to article at publishers website
Database assignments for author(s): Jørgen Eilenberg, Zhi Ming Yuan, Bjarne Munk Hansen, Niels Bohse Hendriksen

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
molecular biology - genes
genetical engin./transgenic plants

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
Bacillus thuringiensis var. kurstaki (entomopathogen)
Bacillus thuringiensis Cry1A-toxin (entomopathogen)