Experimental and Applied Acarology (2022) 86, 299-312

Lina B. Flor-Weiler, Robert W. Behle, Fred J. Eller, Ephantus J. Muturi and Alejandro P. Rooney (2022)
Repellency and toxicity of a CO₂-derived cedarwood oil on hard tick species (Ixodidae)
Experimental and Applied Acarology 86 (2), 299-312
Abstract: The repellency and toxicity of a CO₂-derived cedarwood oil (CWO) was evaluated against actively questing unfed nymphs of four species of hard ticks: Amblyomma americanum (L.), Dermacentor variabilis (Say), Ixodes scapularis Say, and Rhipicephalus sanguineus (Latreille). Using a vertical climb bioassay for repellency, nymphs of these species avoided a CWO-treated filter paper in proportional responses to treatment concentrations. At 60 min of exposure, I. scapularis nymphs were most sensitive with 50% repellency concentration (RC₅₀) of 19.8 µg cm^-2, compared with RC₅₀ of 30.8, 83.8 and 89.6 µg cm^-2 for R. sanguineus, D. variabilis and A. americanum, respectively. Bioassays determined the lethal concentration for 50% (LC₅₀) and 90% (LC₉₀) mortality of nymphs exposed to CWO in treated vials after 24- and 48-h exposure. After 24 h exposure, the LC₅₀ values were 1.25, 3.45 and 1.42 µg cm^-2 and LC₉₀ values were 2.39, 7.59 and 4.14 µg cm^-2 for D. variabilis, I. scapularis and R. sanguineus, respectively, but had minimal effect on A. americanum. After 48 h exposure, the LC₅₀ values were 4.14, 0.78, 0.79 and 0.52 µg cm^-2, and LC₉₀ values were 8.06, 1.48, 1.54 and 1.22 µg cm^-2 for A. americanum, D. variabilis, I. scapularis and R. sanguineus, respectively. The repellency of CWO on tick species decreased with time. The repellency and toxicity bioassays demonstrated concentration-dependent responses of tick nymphs to the oil, indicating the potential of the CO₂-derived cedarwood oil be developed as an eco-friendly repellent and/or acaricide.
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Database assignments for author(s): Robert W. Behle, Fred J. Eller, Ephantus J. Muturi

Research topic(s) for pests/diseases/weeds:
control - general

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Ixodes scapularis
	Rhipicephalus sanguineus
	Amblyomma americanum
	Dermacentor variabilis