Experimental and Applied Acarology (2011) 54, 33-39

K.S. Kubo, V.M. Novelli, M. Bastianel, E.C. Locali-Fabris, R. Antonioli-Luizon, M.A. Machado and J. Freitas-Astúa (2011)
Detection of Brevipalpus-transmitted viruses in their mite vectors by RT-PCR
Experimental and Applied Acarology 54 (1), 33-39
Abstract: The diagnosis of plant diseases caused by Brevipalpus-transmitted viruses (BrTVs) has been done through the analyses of symptoms, transmission electron microscopy, and RT-PCR of infected plant tissues. Here, we report the detection of Citrus leprosis virus C, Orchid fleck virus, Clerodendrum chlorotic spot virus and Solanum violaefolium ringspot virus in their viruliferous vectors Brevipalpus spp. using specific primer pairs for each of the viruses. The efficiency of virus transmission by Brevipalpus mites is low, so the detection of these pathogens in their vectors could constitute an important tool for studies involving virus-vector relationships, transmission, and monitoring the pathogen prior to the appearance of symptoms in the field.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Juliana Freitas-Astua, Marcos Antonio Machado, Valdenice M. Novelli, Karen Sumire Kubo

Research topic(s) for pests/diseases/weeds:
transmission/dispersal of plant diseases

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Orchid fleck dichorhavirus
	Cilevirus leprosis