European Journal of Plant Pathology (2022) 162, 995-1003

Wendy Serra, María B. Lugo Alvarez, Dariel García Rodríguez, Eugenio Alonso-Oliva, Amalia Sanz Llorente, Vladimiro Guarnaccia, Elma Carstens, Jeffrey A. Rollins, Sofie Thijs, Jaco Vangronsveld and Ana M. Manzano León (2022)
Polyphasic identification and MAT1-2 isolates of Phyllosticta citricarpa in Cuba
European Journal of Plant Pathology 162 (4), 995-1003
Abstract: Citrus black spot (CBS), caused by Phyllosticta citricarpa (McAlpine) van der Aa, is a disease that affects Citrus spp. causing production losses, high costs associated with management, and constraints on the fresh market trade. The present investigation aimed to confirm the identity of the CBS pathogen as P. citricarpa and determine its mating-type distribution in Cuba. Phyllosticta isolates were collected from major Cuban citrus-producing provinces (Matanzas, Cienfuegos, Camagüey, and Santiago de Cuba). P. citricarpa and the endophyte P. capitalensis (Henn.) were identified by polyphasic taxonomy, integrating morpho-physiological characteristics with the phylogenetic analysis of ITS, actA, LSU and tef1 loci. Mating-type (MAT) analysis revealed that only the MAT1-2 idiomorph of P. citricarpa is present in Cuba, providing evidence of an atypical CBS disease cycle. These results contribute to a better understanding of the epidemiology of CBS in Cuba and the improvement of CBS management.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): María E. Alvarez, Vladimiro Guarnaccia, Elma Carstens

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution
surveys/sampling/distribution

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Phyllosticta citricarpa	Citrus (genus)	Cuba