European Journal of Plant Pathology (2021) 161, 579-591
Michael Helmut Hagemann, Ute Born, Elke Sprich, Luitgardis Seigner, Hans Oechsner, Benedikt Hülsemann, Jörg Steinbrenner, Patrick Winterhagen and Erich Lehmair (2021)
Degradation of Hop latent viroid during anaerobic digestion of infected hop harvest residues
European Journal of Plant Pathology 161 (3), 579-591
Abstract: The citrus bark cracking viroid (CBCVd) was identified as causal agent for a severe stunting disease in hops. Viroids are highly stable parasitic RNAs, which can be easily transmitted by agricultural practices. Since CBCVd has recently been detected in two European countries a growing concern is that this pathogen will further spread and thereby threaten the European hop production. Biogas fermentation is used to sanitize hop harvest residues infected with pathogenic fungi. Consequently, the aim of this study was to test if biogas fermentation can contribute to viroid degradation at mesophilic (40 °C) and thermophilic (50 °C) conditions. Therefore, a duplex reverse transcription real-time PCR analysis was developed for CBCVd and HLVd detection in biogas fermentation residues. The non-pathogenic hop latent viroid (HLVd) was used as viroid model for the pathogenic CBCVd. The fermentation trials showed that HLVd was significantly degraded after 30 days at mesophilic or after 5 days at thermophilic conditions, respectively. However, sequencing revealed that HLVd was not fully degraded even after 90 days. The incubation of hop harvest residues at different temperatures between 20 and 70 °C showed that 70 °C led to a significant HLVd degradation after 1 day. In conclusion, we suggest combining 70 °C pretreatment and thermophilic fermentation for efficient viroid decontamination.
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Database assignments for author(s): Luitgardis Seigner
Research topic(s) for pests/diseases/weeds:
control - general
Pest and/or beneficial records:
Beneficial | Pest/Disease/Weed | Crop/Product | Country | Quarant.
|
---|---|---|---|---|
Hop latent viroid | Hop (Humulus lupulus) | |||
Citrus bark cracking viroid | Hop (Humulus lupulus) | Slovenia |