European Journal of Plant Pathology (2019) 155, 1241-1263

Sarah Boyd Lade, Carla Román, Ana Isabel del Cueto-Ginzo, Luis Serrano, Ester Sin, María Angeles Achón and Vicente Medina (2019)
Differential proteomics analysis reveals that Azospirillium brasilense (Sp7) promotes virus tolerance in maize and tomato seedlings
European Journal of Plant Pathology 155 (4), 1241-1263
Abstract: Plant growth-promoting rhizobacteria such as Azospirillum brasilense Sp7 can protect plants against viruses but the molecular basis of this phenomenon is unclear. We therefore used differential proteomics to study two pathosystems in the presence and absence of Sp7 during early vegetative growth: tomato (Solanum lycopersicum L. cv. Boludo)/Potato virus X (PVX, KJ631111)/Sp7, and maize (Zea mays cv. B73)/Maize dwarf mosaic virus (MDMV, AM110558)/Sp7). In the maize/MDMV system, PDQuest revealed significant variations in the levels of 19 proteins compared to uninfected controls, including the upregulation of NADP-dependent malic enzyme as a form of host-specific viral anticipation, causing a simultaneous increase in the abundance of proteins related to photosynthesis and plastid functions. However, 42 proteins varied significantly in the maize/MDMV/Sp7 system, including the upregulation of radical-scavenging enzymes and proteins related to methionine metabolism, the glutathione-ascorbate cycle and photosynthesis, increasing the photosynthetic rate. In the tomato/PVX system, we observed significant variations in the levels of 58 proteins reflecting the disruption of the Calvin-Benson cycle, responses to oxidative stress and the inhibition of photosystem II (PSII) activity. We identified 26 proteins that varied in the tomato/PVX/Sp7 system; PSII and plastid proteins transiently declined but partially recovered over time as the Calvin-Benson cycle was induced to compensate. Sp7 therefore triggers induced systemic resistance in both pathosystems without affecting the virus titer, although it does delay the appearance of MDMV. The role of ribulose-1.5-bisphosphate carboxylase/oxygenase small subunit as a host target for viruses is discussed in both pathosystems.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): María Ángeles Achon, Vicente Medina

Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Maize dwarf mosaic virus	Maize/corn (Zea mays)
	Potato virus X	Tomato (Solanum lycopersicum)
Azospirillum brasilense (antagonist)	Potato virus X	Tomato (Solanum lycopersicum)