European Journal of Plant Pathology (2018) 151, 541-547

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Rodrigo Solci Toloy, Tatiana Mituti, Debora Maria Sansini Freitas, Nathalie Kristine Prado Maluta, Talita Nicola Zocca Silva, João Roberto Spotti Lopes, Alberto Fereres and Jorge Alberto Marques Rezende (2018)
Features of the relationship between Tomato severe rugose begomovirus and Bemisa tabaci MEAM1 reveal that the virus is acquired during a probe lasting only one minute
European Journal of Plant Pathology 151 (2), 541-547
Abstract: Tomato severe rugose virus (ToSRV) is a begomovirus found only in Brazil and has been the prevalent species affecting tomato crops in southwest and midwest of the country. Although the virus is known to be transmitted by Bemisia tabaci MEAM1, this study was undertaken to better characterize the relationship between ToSRV and whiteflies on tomato plants. The minimum acquisition and inoculation access periods, latent period, and virus retention time in the vector were determined. The results of transmission experiments showed that the minimum acquisition and inoculation access periods (AAP and IAP) for transmission of ToSRV by MEAM1 were 1 min and 5 min, respectively. After monitoring 50 individual adult whiteflies during a one-minute AAP on ToSRV-infected tomato leaves, 21 insects (42%) were able to acquire the virus, as confirmed by PCR. In contrast, one hundred insects that were individually allowed one-minute AAP on Tomato chlorosis virus (ToCV)-infected tomato leaves, which is a phloem-limited crinivirus, were unable to acquire the virus. The latent period of ToSRV in MEAM1 was 12–15 h, with adult whiteflies retaining the virus for 25 days. Although the tissue distribution of ToSRV in tomato has not been determined, the short AAP suggests that the virus may be present in mesophyll cells, from where it may be acquired during a one-minute feeding probe.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Jorge A.M. Rezende, João Roberto Spotti Lopes, Alberto Fereres, Nathalie Kristine Prado Maluta

Research topic(s) for pests/diseases/weeds:
transmission/dispersal of plant diseases

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.

Bemisia tabaci biotype MEAM1 Tomato (Solanum lycopersicum) Brazil (south)
Tomato severe rugose virus Tomato (Solanum lycopersicum) Brazil (south)