European Journal of Plant Pathology (2015) 143, 11-23

Chunxian Chen, Clive H. Bock, Mike H. Hotchkiss, Matteo M. Garbelotto and Ted E. Cottrell (2015)
Observation and identification of wood decay fungi from the heartwood of peach tree limbs in central Georgia, USA
European Journal of Plant Pathology 143 (1), 11-23
Abstract: Peach tree health, longevity, and limb strength can be affected by wood decay fungi, but the extent of the colonization and their identities have not been characterized in Georgia, United States of America. In an incubation experiment, dense white fungal mycelium grew on the cross-cut faces of asymptomatic limb sections sampled from peach scion cultivars, but no mycelium grew on those from rootstock cultivars. Among the scion cultivars, a Chi-square analysis indicated significant differences in the incidence of colonization. A dissection of six broken peach tree scaffold limbs showed symptoms of colonization along the entire length of the limbs. Only 41 % of primary branches had symptoms of wood decay, but no secondary branches appeared to have symptoms. Next generation sequencing (NGS) and metagenomic analysis of a combined DNA sample revealed the contigs were aligned to sequences of Trametes versicolor and/or Schizophyllum commune, suggesting these two white-rot fungi were present in the sample tested. Sanger sequencing confirmed two fragments of the same size but distinct nucleotide peak intensities were amplified from the sample used for NGS by two pairs of primers, providing supporting evidence that the two fungi were in the bulk sample but might differ in abundance or incidence. Subsequent multiplexing polymerase chain reaction diagnostics of ten scion samples confirmed the dominance of Trametes spp. (all ten samples), although Stereum spp. (five samples), Schizophyllum spp. (three samples) and Hericium spp, (one sample) were sometimes found coexisting with Trametes spp. Future research needs on this peach-fungus association are discussed.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Ted E. Cottrell

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Schizophyllum commune	Peach/nectarine (Prunus persica)	U.S.A. (SE)
	Trametes versicolor	Peach/nectarine (Prunus persica)	U.S.A. (SE)