European Journal of Plant Pathology (2011) 130, 287-295

Ruba Abuamsha, Mazen Salman and Ralf-Udo Ehlers (2011)
Effect of seed priming with Serratia plymuthica and Pseudomonas chlororaphis to control Leptosphaeria maculans in different oilseed rape cultivars
European Journal of Plant Pathology 130 (3), 287-295
Abstract: The efficacy of a seed treatment of oilseed rape (OSR) (Brassica napus) with the rhizobacteria Serratia plymuthica (strain HRO-C48) and Pseudomonas chlororaphis (strain MA 342) applied alone or in combination against the blackleg disease caused by Leptosphaeria maculans was tested with different cultivars. Seeds were soaked in bacterial suspensions (bio-priming) to obtain log₁₀ 6-7 CFU seed⁻¹. Cotyledons were inoculated with a 10 ul droplet of L. maculans spore suspension of log₁₀ 7 spores ml⁻¹ and the disease index (size of lesions) was evaluated 14 days later. A mean disease reduction of 71.6% was recorded for S. plymuthica and of 54% for P. chlororaphis. The combined treatment was not superior to the treatment with S. plymuthica alone. The reduction of the disease caused by S. plymuthica was independent of the cultivar's susceptibility, whereas the control effect recorded with P. chlororaphis increased with decreasing cultivar resistance to blackleg disease. The bacterial colonization of OSR was restricted to the roots and hypocotyl. No significant difference in bacterial colonization of the rhizosphere was observed between different cultivars, nor between single or combined bacterial seed treatments.
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Link to article at publishers website
Database assignments for author(s): Ralf-Udo Ehlers

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
evaluation - screening - selection
application technology

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Plenodomus lingam	Rape/canola (Brassica napus)
Pseudomonas chlororaphis (antagonist)	Plenodomus lingam	Rape/canola (Brassica napus)
Serratia plymuthica (antagonist)	Plenodomus lingam	Rape/canola (Brassica napus)