European Journal of Plant Pathology (2009) 124, 127-132
B.Y. Lee and K.H. Ryu (2009)
Incidence of virus diseases and RT-PCR detection of Daphne -infecting viruses in Korea
European Journal of Plant Pathology 124 (1), 127-132
Abstract: Reverse transcription-polymerase chain reaction (RT-PCR) was developed for the detection of viruses from Daphne odora plants in this study. Five sets of primers were designed for the specific detection of five different viruses, Daphne virus Y (DVY, Potyvirus), Cucumber mosaic virus (CMV, Cucumovirus), Daphne virus S (DVS, Carlavirus), Cycas necrotic stunt virus (CNSV, Nepovirus), and Watermelon mosaic virus (WMV, Potyvirus) in collected Daphne plants. RT-PCR could successfully detect the viruses from the Daphne plants. Two potyviruses (DVY and WMV), CNSV, CMV and DVS were frequently detected in >85% samples examined in this study. CNSV was the most prevalent virus (78%), and the infection rate for the three viruses WMV, CMV and DVS was >60%. These results demonstrate that virus diseases on Daphne plants are extremely severe and widespread in Korea. It is especially notable that mixed virus infections with more than three different viruses were very common and 12% of Daphne plants were infected by the five viruses. The results suggest that five primer sets developed in this study can successfully detect the target viruses and can be useful for the monitoring of virus incidence and indexing of the virus-free Daphne plants.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Ki Hyun Ryu
Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution
Pest and/or beneficial records:
| Beneficial | Pest/Disease/Weed | Crop/Product | Country | Quarant.
|
|---|---|---|---|---|
| Cucumber mosaic virus | Daphne (crop) | Korea-South | ||
| Watermelon mosaic virus | Daphne (crop) | Korea-South | ||
| Nepovirus cycas | Daphne (crop) | Korea-South | ||
| Daphne virus S | Daphne (crop) | Korea-South | ||
| Daphne virus Y | Daphne (crop) | Korea-South |
