European Journal of Plant Pathology (2007) 117, 71-79

M.-N. Douaiher, E. Nowak, V. Dumortier, R. Durand, Ph. Reignault and P. Halama (2007)
Mycosphaerella graminicola produces a range of cell wall-degrading enzyme activities in vitro that vary with the carbon source
European Journal of Plant Pathology 117 (1), 71-79
Abstract: In this study, two Mycosphaerella graminicola isolates produced a range of cell wall-degrading enzymes (CWDE) in vitro that can potentially degrade wheat cell walls. The influence of three carbon sources on CWDE in vitro production was tested: 1) 1 % galactose (w/v), 2) 1% wheat cell walls (w/v) and 3) a mixture of 1% galactose (w/v) and 1% wheat cell walls (w/v). Six major activities produced by both isolates were detected: xylanase, ß-1,3-glucanase, polygalacturonase, cellulase, ß-xylosidase and ß-galactosidase. Time-course experiments showed that different levels of enzyme activities were obtained with isolates 323 and 94269. These activities levels varied also with the type of carbon source used. Principal Component Analysis showed that the enzyme activities are gathered into two groups. None of the activities of the first group was correlated to the activities of the second group. It also showed that the optimal medium that allowed the production of most of the major activities contained both galactose and wheat cell walls.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Patrice Halama, Philippe Reignault

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Zymoseptoria tritici