European Journal of Plant Pathology (2004) 110, 573-585
T. Yli-Mattila, S. Paavanen-Huhtala, P. Parikka, P. Konstantinova and T.Y. Gagkaeva (2004)
Molecular and morphological diversity of Fusarium species in Finland and north-western Russia
European Journal of Plant Pathology 110 (5-6), 573-585
Abstract: In 2001 the range of the total Fusarium contamination percentage of infected seeds was between 0% and 44%, while in 2002 the contamination level was 2-25% in naturally infected Finnish samples and 5-14.5% in six samples from northwestern Russia. The most common Fusarium species in barley were F. avenaceum, F. arthrosporioides, F. sporotrichioides and F. culmorum, while in spring wheat the most common Fusarium species were F. avenaceum, F. arthrosporioides, F. culmorum, F. sporotrichioides and F. graminearum. In most cases, molecular identification with species-specific primers corresponded to the morphological analyses and allowed the identification of degenerated and otherwise morphologically difficult cultures. It was even possible to separate most of the F. arthrosporioides isolates from Finland from the closely-related F. avenaceum isolates. In the phylogenetic analysis of combined ß-tubulin, IGS and ITS sequences most European F. arthrosporioides formed a separate clade from most isolates of F. avenaceum and from all isolates of F. tricinctum. Most of the species-specific primers also amplified DNA extracted from grain samples. It was, for instance, possible to detect F. avenaceum in all barley samples with contamination levels higher than 1% and in all spring wheat samples with contamination levels higher than 3%. The detection level for F. graminearum was at a contamination level of 3-5% and that for F. culmorum at a contamination level of 1-5%. In addition, the first Finnish F. langsethiae isolate was found by means of species-specific primers.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Sari Paavanen-Huhtala, Tatiana Yu. Gagkaeva
Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution
surveys/sampling/distribution
