European Journal of Plant Pathology (2001) 107, 457-465

Yeshitila Degefu, Lars Paulin and Peter Stephensen Lübeck (2001)
Cloning, sequencing and expression of a xylanase gene from the maize pathogen Helminthosporium turcicum
European Journal of Plant Pathology 107 (4), 457-465
Abstract: A gene encoding an endoxylanase from the phytopathogenic fungus Helminthosporium turcicum Pass. was cloned and sequenced. The entire nucleotide sequence of a 1991 bp genomic fragment containing an endoxylanase gene was determined. The xylanase gene of 795 bp, interrupted by two introns of 52 and 62 bp, encoded a protein of 227 amino acids showing up to 95% amino acid homology with other fungal xylanases. The precise splicing site of the introns was identified by sequencing the corresponding cDNA. A northern blot showed that the gene is expressed when the fungus is grown in a medium containing xylan as a sole carbon source. The cloned xylanase gene was expressed in maize plants during infection.
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Link to article at publishers website
Database assignments for author(s): Yeshitila Degefu

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Exserohilum turcicum	Maize/corn (Zea mays)