European Journal of Plant Pathology (1999) 105, 191-199

B.Z. Meng, R. Johnson, S. Peressini, P.L. Forsline and D. Gonsalves (1999)
Rupestris stem pitting associated virus-1 is consistently detected in grapevines that are infected with rupestris stem pitting
European Journal of Plant Pathology 105 (2), 191-199
Abstract: Rupestris stem pitting (RSP) appears to be the most widespread disease of the rugose wood complex of grapevines. In previous work we showed that rupestris stem pitting associated virus-1 (RSPaV-1), an agent that has similarities in genome structure to apple stem pitting virus is associated with RSP (Meng et al., 1997, 1998). To validate the association of RSPaV-1 with RSP, grapevines that had been indexed on the RSP indicator St. George (Vitis rupestris Scheele) were collected from four major grape-growing countries and tested for RSPaV-1 by reverse transcription-polymerase chain reaction (RT-PCR) assay. Depending on the sets of primers used, we detected RSPaV-1-specific sequences from 85% to nearly 100% of the grapevines that had indexed positive for RSP. These data further demonstrate that RSPaV-1 is closely associated with, and likely to be the causal agent of RSP. We have also shown that RT-PCR is a fast and reliable alternative to biological indexing for the diagnosis of RSP.
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Link to article at publishers website
Database assignments for author(s): Baozhong Meng

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Grapevine rupestris stem pitting-associated virus	Grapevine (Vitis)