European Journal of Entomology (2001) 98, 189-194

Neelam Narang, Franck Hérard, Edward M. Dougherty, Kim Chen and Fernando E. Vega (2001)
A gypsy moth (Lymantria dispar, Lepidoptera: Lymantriidae) multinucleocapsid nuclear polyhedrosis virus from France: comparison with a North American and a Korean strain
European Journal of Entomology 98 (2), 189-194
Abstract: As part of a search for natural enemies of the gypsy moth (Lymantria dispar), virus-infected samples were collected near Toulouse, France. Light and electron microscope studies confirmed that the French strain is a multinucleocapsid nuclear polyhedrosis virus (MNPV). In vivo bioassays using the New Jersey strain of L. dispar, and comparing L. dispar MNPV (LdMNPV) strains from France, North America and Korea, showed that the French strain was the least active, whereas the North American strain had the highest activity. The viral efficacy of all strains was enhanced 200 to 1300-fold in the presence of 1% fluorescent brightener. The enhancement was highest in the American strain and lowest in the French strain. French LdMNPV (LdMNPVF) DNA cut with four restriction enzymes (BamHl, EcoRl, Hindlll, and Notl) revealed minor fragment size differences, but many similarities when compared to the North American and the Korean strain. PCR amplification of a LdMNPV early gene (G22) was detected in the North American and the Korean strain, but not in the French strain.
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Database assignments for author(s): Franck Herard

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Lymantria dispar	Oak (Quercus)	France
Alphabaculovirus lydisparis (entomopathogen)	Lymantria dispar	Oak (Quercus)	France