Euphytica (2013) 194, 197-205

Meng-Ping Lei, Guang-Rong Li, Li Zhou, Cheng-Hui Li, Cheng Liu and Zu-Jun Yang (2013)
Identification of wheat-Secale africanum chromosome 2R^afr introgression lines with novel disease resistance and agronomic characteristics
Euphytica 194 (2), 197-205
Abstract: The wild species, Secale africanum Stapf., serves as a valuable germplasm resource for increasing the diversity of cultivated rye (Secale cereale L.) and providing novel genes for wheat improvement. The objective of this study was to identify new wheat-S. africanum chromosome 2R^afr derivatives by molecular markers and in situ hybridization, and to test the effects of centric translocations of S. africanum chromosome 2R^afr on disease resistance and agronomic performance of wheat. The T2R^afrS.2DL and T2DS.2R^afrL translocations, and other S. africanum chromosome introgressions such as a 2R^afr substitution as well as monotelo-2R^afrS and 2R^afrL addition lines, were identified by the genomic in situ hybridization and sequential fluorescence in situ hybridization (FISH). Twenty-three molecular markers were localized on chromosome 2R^afr arms which will facilitate future identification of their introgressions into wheat. A comparison of FISH patterns and the molecular marker distribution between the S. africanum chromosome 2R^afr and cultivated rye chromosome 2R indicates differentiation between the wild and cultivated Secale genomes. Tests of disease resistance and agronomic performance of wheat-S. africanum chromosome 2R^afr derivatives indicated that chromosome 2R^afrL carried gene(s) for dwarfing and stripe rust resistance, while the 2R^afrS translocation line was the most favorable for agronomic performance when compared with the substitution and 2R^afrL translocation lines. These results encourage the continued use of novel wheat-S. africanum 2R^afr derivative lines in wheat improvement.
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Link to article at publishers website

Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Puccinia striiformis	Wheat (Triticum)