Environmental Microbiology (2002) 4, 819-823

Diana Stjepandic, Christian Weinel, Helmut Hilbert, Hean L. Koo, Frank Diehl, Karen E. Nelson, Burkhard Tümmler and Jörg D. Hoheisel (2002)
The genome structure of Pseudomonas putida: high-resolution mapping and microarray analysis
Environmental Microbiology 4 (12), 819-823
Abstract: As part of a collaborative project aimed at sequencing and functionally analysing the entire genome of Pseudomonas putida strain KT2440, a physical clone map was produced as an initial resource. To this end, a high-coverage cosmid library was arrayed and ordered by clone hybridizations. Restriction fragments generated by rare-cutting enzymes and plasmids containing the rrn operon and 23S rDNA of Pseudomonas aeruginosa were used as probes and, parts of the cosmids were end-sequenced. This provided the information necessary for merging and comparing the macro-restriction map, cosmid clone order and sequence information, thereby assuring co-linearity of the eventual sequence assembly with the actual genome. A tiling path of clones was selected, from the shotgun clones used for sequencing, for the production of DNA microarrays that represent the entire genome including its non-coding portions.
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Link to article at publishers website

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
Pseudomonas putida (antagonist)