Entomological Research (2011) 41, 298-299

Zhen Ji, Do Young Kim, Han-Young Cho, Yi-Joon Kim, Dong-Ha Shin, Kwang-Hee Son and Ho-Yong Park (2011)
Influences of medium composition on mycelial growth, sporulation and chitinase secretion of Metarhizium anisopliae HY-2
Entomological Research 41 (6), 298-299
Abstract: Metarhizium anisopliae HY-2, an entomopathogenic fungus isolated from pest cadaver in South Korea, is applicable as a potent bio-pesticide because of its insecticidal activity to Adoretus tenuimaculatus. Here we report this strain varied in its ability to grow under different nutritional and environmental factors. Temperature at 28°C and pH from 6 to 7 were obtained the best-fit environmental requirements. Insect pupa and tryptone were found the optimal nitrogen sources for both mycelial growth and sporulation. The strain reached the most suitable effects at the carbon-to-nitrogen ratio from 10:1 to 20:1 when the carbon concentration was 4 g/ml provided by glucose and nitrogen supplied by tryptone. Additionally, strain HY-2 produced an extracellular chitinase at a relatively high level (21.1 mU/ml) when cultivated for 20 days on a medium supplemented with colloidal chitin (1%) and insect pupa (0.5%), indicating that synthesis of chitinase is subject to induction by certain substrate.
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Link to article at publishers website

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
rearing/culturing/mass production
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Adoretus tenuimaculatus		Korea-South
Metarhizium majus (entomopathogen)	Adoretus tenuimaculatus		Korea-South