Entomologia Sinica (1995) 2, 239-246

Dunlun Song, Zuorui Shen, Yahua Chen, Ziduo Liu and Ziniu Yu (1995)
Cloning and expression of CryIVD gene of insecticidal crystal protein of Bacillus thuringiensis in the acrystalliferous strain
Entomologia Sinica 2 (3), 239-246
Abstract: The plasmid pGEMl, carrying CryIVD, CytA and 20-kDa protein genes, was extracted and digested with Hind II /BamH I. A 5.4 kb fragment containing Cry IV D and 20-kDa protein gene was purified and ligated to an E. coli TG1. The recombinant plasmid was analyzed by restriction map and Southern blotting to determine the correct insert. Then the recombinant was transferred into Bacillus thuringiensis acrystalliferous mutant Bti. IPS. 78/11 by electroporation. The clones with strong expressiveness were obtained. The engineered strains express 72-kDa and 20-kDa proteins and form irregular hexagon paraspore crystal. One strain bioassayed is very toxic to Culex fatigans larvae with LC₅₀ of 0.53 µg/ml.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Dun-Lun Song, Zi Niu Yu

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
molecular biology - genes
genetical engin./transgenic plants

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Culex quinquefasciatus
Bacillus thuringiensis Cry4D-toxin (entomopathogen)	Culex quinquefasciatus