Entomologia Experimentalis et Applicata (2007) 122, 53-58

Fangneng Huang, B.Rogers Leonard, Donald R. Cook, Donna R. Lee, David A. Andow, Jack L. Baldwin, Kelly V. Tindall and Xiaoyi Wu (2007)
Frequency of alleles conferring resistance to Bacillus thuringiensis maize in Louisiana populations of the southwestern corn borer
Entomologia Experimentalis et Applicata 122 (1), 53-58
Abstract: Transgenic maize [Zea mays L. (Poaceae)] expressing Bacillus thuringiensis proteins (Bt maize) has become the most important tool for managing stalk borers in maize in the USA. The current strategy for delaying the evolution of resistance in target insects for Bt maize is referred to as high dose/refuge strategy. A key requirement of the strategy is that initial resistance allele frequencies in field insect populations are low (e.g., <0.001). More than 200 iso-line families of the southwestern corn borer, Diatraea grandiosella Dyar (Lepidoptera: Crambidae), a major target stalk borer pest of Bt maize, were developed from Louisiana populations and evaluated for Bt resistance using a modified F₂ screening method during 2005. No major resistance alleles were detected in these populations. The results showed that the expected Bt resistance allele frequency in the Louisiana populations was <0.0035 with 95% probability and a detection power of 83.9 ± 0.6%. The F₂ screen indicates that Bt resistance allele frequencies in D. grandiosella are low among the Louisiana populations and should meet the rare resistance allele requirement of the 'high dose/refuge' strategy.
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Link to article at publishers website
Database assignments for author(s): Fang Neng Huang

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
resistance/tolerance/defence of host

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Diatraea grandiosella	Maize/corn (Zea mays)	U.S.A. (mid S)
Bacillus thuringiensis genes in crops (entomopathogen)	Diatraea grandiosella	Maize/corn (Zea mays)	U.S.A. (mid S)