EPPO Bulletin (2000) 30, 559-562
L. Schena, M.M. Finetti Sialer and D. Gallitelli (2000)
Specific identification of Aureobasidium pullulans strain L47 using Scorpion PCR
EPPO Bulletin 30 (3-4), 559-562
Abstract: Strain L47 of Aureobasidium pullulans is an effective biocontrol agent for several post-harvest fruit diseases, even when applied in the field before harvesting. This strategy requires a specific method of monitoring in order to optimize formulations and to evaluate the level of colonization and dispersal in the environment. We have detected strain L47 among other morphologically indistinguishable isolates of A. pullulans using Scorpion PCR, an automated real-time fluorescence detection system that targets a 145-bp fragment of the L47 genome. We propose this approach as a specific, rapid and reliable method of monitoring A. pullulans, strain L47.
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Link to article at publishers website
Database assignments for author(s): Leonardo Schena
Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
identification/taxonomy
Pest and/or beneficial records:
Beneficial | Pest/Disease/Weed | Crop/Product | Country | Quarant.
|
---|---|---|---|---|
Penicillium digitatum | ||||
Aureobasidium pullulans (antagonist) | Penicillium digitatum |