EPPO Bulletin (2000) 30, 381-383

S.A. Weller, J.G. Elphinstone, N.C. Smith and D.E. Stead (2000)
Detection of Ralstonia solanacearum from potato tissue by post-enrichment TaqMan PCR
EPPO Bulletin 30 (3-4), 381-383
Abstract: TaqMan polymerase chain reaction (PCR) exploits the 5'-nuclease activity of Taq DNA polymerase in conjunction with fluorogenic DNA probes. A positive PCR reaction results in a measurable emission of fluorescence within 2h. TaqMan assays for several plant-pathogenic bacteria have been designed, including the potato brown rot pathogen Ralstonia solanacearum. Enrichment techniques designed to improve the detection of low pathogen populations in potato tissue are also suitable for use with TaqMan PCR. The quantitative aspect of TaqMan PCR enables relative population level differences between pre-enriched and enriched samples to be demonstrated and thus cell viability to be evaluated.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Neil C. Smith, John Elphinstone

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Ralstonia solanacearum