Current Microbiology (2005) 51, 262-266

Milena de P.M.A. Ribeiro, Marina de T.F. Dellias, Siu M. Tsai, Anne Bolmströn, Lyndel W. Meinhardt and Cláudia de M. Bellato (2005)
Utilization of the Etest assay for comparative antibiotic susceptibility profiles of citrus variegated chlorosis and Pierce's disease strains of Xylella fastidiosa
Current Microbiology 51 (4), 262-266
Abstract: Xylella fastidiosa has a wide host range. Isolates of this bacterium that cause diseases in citrus (CVC) and grapes (PD) share 98% genome homology, and 95.7% amino acid identity. Drug resistance genes show a higher level of divergence and may be involved in the X. fastidiosa-host interaction. Antibiotic susceptibility of CVC and PD strains were compared utilizing the Etest strip method (AB Biodisk). Etest is applicable for fastidious slow-growing organisms due to its reproducibility. Results showed that the CVC strain was resistant to bacitracin, cefotaxime, and trimethoprim, and susceptible to chloramphenicol, erythromycin, gentamicin, kanamycin, streptomycin, and tetracycline. The PD strain was susceptible to all tested antibiotics, except kanamycin and trimethoprim. Both isolates produced a class C ß-lactamase. These data support previous antibiotic studies and gene discrepancies found in the sequencing data of PD and CVC strains. These results demonstrate the efficacy of utilizing Etest assays for X. fastidiosa strains.
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Link to article at publishers website
Database assignments for author(s): Lyndel W. Meinhardt

Research topic(s) for pests/diseases/weeds:
control - general
pesticide resistance of pest

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Xylella fastidiosa