Chinese Journal of Entomology (1995) 15, 333-344

Cheng-Jen Shih and Ren-Wei Lin (1995)
[Establishment of a cell line from pupal ovaries of Spodoptera litura (Lepidoptera : Noctuidae)]
Chinese Journal of Entomology 15 (4), 333-344
Abstract: A cell line was established from pupal ovaries of the tobacco cutworm, Spodoptera litura. This cell line, designated as SL7A, was grown in TNM-FH insect cell culture medium supplemented with 10% fetal bovine serum. In primary cultures, small numbers of wandering connective-tissue-like cells appeared. After subculturing, cells generally became spherical rather than spindle shaped and adhered to the bottom of the culture plate, forming a monolayer of cells. The growth curve of the cells was Y = e^{-0.6736+0.0323X}, r² = 0.98. The population doubling time during logarithmic growth at 28 °C was 21.4 h. The saturated population of cells was approximately 2X10⁷ cells in a 25-T flask, representing a 20-fold increase over the initial population in 4 days. Karyotype analysis revealed that the nucleus of SL7A cells contained numerous microchromosomes. The number of chromosomes appeared to follow a normal distribution in the range from 63 to 382 with a mean of 215.5±74.3. Isozyme analysis revealed that the SL7A cell line was characteristically different from 4 insect cell lines.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Chinese)

Research topic(s) for pests/diseases/weeds:
rearing/culturing/mass production

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Spodoptera litura